BES - Aquatic Toxicity Testing Results for Soilworks, LLC - Three 96-Hour Product LC-50 Bioassays

Nanette Malan Lake, Laboratory Manager; David Block, Laboratory Director
2005-10-06
Block Environmental Services, Inc.

BLOCKENVIRONMENTAL SERVICES

 

2451 Estand Way

Pleasant Hill, CA 94523-3911 (925) 682-7200 FAX 686-0399

 

 

 

Aquatic Toxicity Testing Results for Soilworks, LLC Three 96-Hour Product LC-50 Bioassays

 

 

 

 

Prepared For:

Chad Falkenberg Soilworks, LLC

681 N. Monterey St., Ste 101

Gilbert, AZ 85233-3818

 

 

 

BES Sample #22382

 

 

 

 

Prepared By:

Block Environmental Services, Inc.

2451 Estand Way Pleasant Hill, CA 94523

 

 

October 6, 2005

 

 

David Block, Ph.D

Laboratory Director

Nanette Malan Lake

Laboratory Manager

 

 

  1. 1.     INTRODUCTION

 

 

Soilworks of Gilbert, Arizona requested Block Environmental Services (BES) to perform 96-Hr LC-50 aquatic bioassays, using rainbow trout (Oncorhynchus mykiss), fathead minnows (Pimephales promelas) and the mysid (Americamysis bahia) for the product Durasoil™, a synthetic organic dust control agent.

 

BES received the product on July 25, 2005 and performed the definitive toxicity tests in order to determine concentration-response curves for fish mortality, LC-S0's and the 95 percent confidence inte rvals.

 

A definitive test was performed for each species in order to estimate a concentration range within which the LC-50 value would fall. The definitive tests consisted of 5 concentrations and a laboratory control, run in duplicate.

 

The test protocols and guidelines followed the EPA Ecological Effects Test Guidelines (OPPTS 850.1075 Fish Acute Toxicity Test, Freshwater and Marine).

 

As a QNQC measure, standard reference toxicant tests were run to confirm good organism quality .

 

This report describes the procedures used and the results obtained for the aquatic toxicity tests initiated on July 27, 2005 and completed August 20, 2005.

 

BES is an Environmental Laboratory Accreditation Program certified laboratory (#1812).

continue reading...

BES# 22382                                                                                                                 Page 2 of 8

 

  1. 2.    MATERIALS AND METHODS                                                                                           II

2.1    Sample and Test Preparation

 

Sample identification - Durasoil™

Chemical name: Synthetic Organic Dust Control Agent Appearance and odor: Colorless, odorless

Solubility: Insoluble in water

 

 

The sample was received on July 25, 2005 and kept at ambient room temperature for the duration of the study.

 

Samples to set up the concentration series for the range finding tests were weighed out using a Sartorius Balance, disposable dispenser pipettes and clean glassware. The concentration series for the range finding tests are summarized in Table 1.

 

A summary of testing parameters by organism can be found in Table 2.

 

 

2.2   Organism information

 

Species:         Rainbow trout (Oncorhynchus myldss)

Supplier:       In house culture Stock date: 07/05/2005

Age:               Juvenile(< 3.0g)

 

Species:        Fathead minnow (Pimephales promelas)

Supplier:       Commercial supplier Stock date: 07/20/2005

 

Age:

 

Species:


Juvenile(< 3.0g)

 

Mysid (Americamysis bahia)

 

Supplier :       Commercial supplier Stock date: 08/16/2005

Age:               4 days old

 

 

BES# 22382                                                                                                                 Page 3 of 8

 

 

 

 

2.3     Reference Toxicant

 

 

Sodium Dodecyl Sulfate was used as the reference toxicant for the rainbow trout and fathead minnows. . Potassium chromate was used as the reference toxicant for the mysid.   The toxicity endpoints from the reference toxicant tests are subsequently plotted on a running control chart from the last 20 tests. The mean values as well as the upper and lower control limits(± 2 standard deviations) are recalculated with each successive test result.   The outliers, which are values falling outside the upper and lower control limits, and trends of increasing or decreasing sensitivity, are readily identified.

 

  1. 3.    TOXICITY TEST PROCEDURES

 

 

3.1    Test Procedures

 

A detailed procedure for each test is outlined in laboratory standard operating procedures (SOP's) kept at the BES laboratory and are based upon the following references:

 

  • Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms, Sth Edition (EPA/821/R-02/012).

 

Additional guidelines used for these tests:

 

 

  • US EPA Ecological Effects Test Guidelines (OPPTS 850.1075 Fish Acute Toxicity Test, Freshwater and Marine).
    • US EPA Ecological Effects Test Guidelines (OPPTS 850.1035 Mysid Acute Toxicity

Test).

 

 

 

 

 

 

 

 

BES# 22382                                                                                                                 Page 4 of8

 

 

 

 

3.2    Data Analysis

 

 

Toxicity testing results were analyzed using the software program ToxCalc Version 5.

 

 

The trimmed Spearman Karber method was used to determine the LC/EC values.   The testing compared the sample responses with the laboratory control water. The parameters of interest are the LC-50 value and the 95% upper and lower confidence limits.

 

 

  1. 4.      TESTINGPARAMETERS

 

 

Table 1: Concentration series for the range finding tests (mg/L).

 

Organism

0. mykiss

P. promelas

A. bahia

Control

Control

Control

1

1

1

10

10

10

100

100

100

1,000

1,000

1,000

10,000

10,000

10,000

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

BES# 22382                                                                                                                 Page 5 of8

 

Table 2:  Summary of Testing Parameters                                                                              II

 

Test Conditions

Organism

0. mykiss

P. promelas

A. bahia

Temperature

12 ± 1 °C

20 ± 1 °C

25 ± 1 °C

Light Intensity

50-100 ft-c

Photoperiod

16hr light: 8hr dark

Test Chamber

6 L glass aquarium

6 L glass aquarium

400 ml glass beaker

Test Solution Volume

SL

SL

200ml

Organism Age

Juvenile(< 3.0g)

Juvenile (< 3.Og)

1-5 days

Organism source

Trout Lodge Inc.,

Sumner WA

Thomas Fish Co.,

Anderson CA

Aquatic Biosystems,

Fort Collins, CO

Organisms/chamber

10

Chamber/ concentration

2

Organisms/concentration

20

Food Source

None

None

Artemia nauplii

Feeding Frequency

None

None

Day O and at 48 hr

Cleaning

None

Aeration

None, unless D.O <

6.0 mg/L

None, unless D.O <

4.0 mg/L

None, unless D.O <

4.0 mg/L

Reference Toxicant

SDS

SDS

K2CrO4

Reference toxicant

concentrations

8, 16, 24, 32 and 40

mg/L

8, 16, 24, 32 and 40

mg/L

1.05, 2.1, 4.2, 8.5 and

16.9 mg/L

Test Duration

96 hr

Effects Measured

Survival

Test Acceptability

2:90% survival in control

 

 

BES# 22382                                                                                                                 Page 6 of 8

 

 

 

 

  1. 5.     RESULTS

 

5.1    Rainbow trout (Oncorhynchus mykiss) results

 

Table 3   Range Fm'  d"mg Test Resu ts

Concentration (mg/L)

Survival (%)

Cl

100

10

100

50

100

100

100

1,000

100

10,000

100

 

5.2   

Concentration (m!dL)

Survival (%)

Cl

100

10

100

50

100

100

100

1,000

100

10,000

100

 

 

Fathead minnow (Pimephales promelas) results Table 4: Range Finding Test Results

 

 

 

 

 

 

5.3    Mysid (Americamysis bahia) results

 

 

Table 5:


Range Fma· mg Test Resu ts

 

Concentration (me/L)

Survival (%)

Cl

100

10

100

50

100

100

100

1,000

100

10,000

100

 

 

5.4     Testing Notes

The Laboratory control water passed the acceptability criteria for survival (2:_90%) for the rainbow trout, fathead minnows and mysid 96 hr bioassays. The reference toxicant test results generated survival and growth endpoints that were within control chart limit ations. These responses indicate that the values elicited for the Durasoil™ product are valid.

 

 

 

BES# 22382                                                                                                                 Page 7 of8

 

 

 

 

  1. 6.     CONCLUSIONS

 

 

The objective of these tests was to determine the toxicological effects (if any) of the product, Durasoil™ on aquatic organisms that represent different thropic levels in the environment. The results for the definitive bioassay tests using the product Durasoil™, received in July 2005 indicate that:

 

  • 0.mykisssurvival was not adversely effected for the product at concentrations up to 10,000 mg/L. Results obtained from the reference toxicant tests are typical of in­ house sensitivity data to NaCl, indicating that the stock organisms that were used were in good condition.
    • P. promelas survival was not adversely effected for the product at concentrations up to 10,000 mg/L. Results obtained from the reference toxicant tests are typical of in­ house sensitivity data to CuSO4 , indicating that the stock organisms that were used were in good condition .
      • A.bahiasurvival was not adversely effected for the product at concentrations up to 10,000 mg/L. Results obtained from the reference toxicant tests are typical  of in­ house sensitivity data to K2CrO4 , indicating that the stock organisms that were used were in good condition

 

 

 

BES# 22382                                                                                                                 Page 8 of 8

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