BES - Aquatic Toxicity Testing Results for Soilworks - One Product LC-50 Bioassay Using Oncorhynchus mykiss

David Block; Ph.D; Laboratory Director; Nanette Bradbury; B.S.; Laboratory Manager
2011-05-23
Soilworks, LLC

BLOCK ENVIRONMENTAL  SERVICES

 

2451 Estand Way

Pleasant Hill, CA 94523-3911 (925) 682-7200 FAX 686-0399

 

 

 

Aquatic Toxicity Testing Results for Soilworks, LLC One Product LC-50 Bioassay using Oncorhynchusmykiss

 

 

 

 

Prepared For: Chad Falkenberg Soilworks, LLC

2450 s. Gilbert Rd., Ste 210

Chandler, AZ 85286

 

 

 

BES Sample # 27649

 

 

 

 

Prepared By:

Block Environmental Services, Inc.

2451 Estand Way Pleasant Hill, CA 94523

 

 

 

May 23, 2011

 

 

 

David Block, Ph.D

Laboratory Director


Nanette Bradbury, B.S.

Laboratory Manager

continue reading...

  1. 1.    INTRODUCTION

 

 

Soilworks of Chandler, Arizona requested Block Environmental Services (BES) to perform a 96- Hr LC-50 aquatic bioassay on the synthetic dust control agent, Durasoil™, using the rainbow trout (Oncorhynchus mykiss).

 

BES received the product on April 13, 2011 and performed a multiconcentration toxicity test in order to determine a concentration-response curve for the mortality and LC-50 endpoint.

 

The multi-concentration toxicity tests consisted of five product concentrations (2000 mg/L, I 000 mg/L, 100 mg/L, 10 mg/L, and I mg/L) and a Laboratory control, run in duplicate.

 

This report describes the procedures used and the results obtained for the aquatic toxicity test initiated on May 12, 2011.

 

BES is an Environmental Laboratory Accreditation Program certified laboratory (#1812).

 

 

 

BES# 27649                                                                                                             Page 2 of 6

 

 

 

  1. 2.    MATERIALS AND METHODS

2.1    SAMPLEANDTESTPREPARATIONSample identification - Durasoil™

Chemical name: Synthetic Organic Dust Control Agent Appearance and odor: Colorless, odorless

Solubility: Insoluble in water

 

 

The sample was received on April 13, 2011 and stored at ambient room temperature for the duration of the study.

 

Samples to set up the definitive tests were weighed out using a Sartorius Balance, disposable dispenser pipettes and clean glassware.

 

A summary of testing parameters by organism can be found in Table 1.

 

 

2.2    TOXICITY TEST PROCEDURES

 

 

2.2.1        Test Procedures -A detailed procedure for this test is outlined in laboratory standard operating procedures (SOPs) kept at the BES laboratory and they are based upon the following reference:

 

  • EPA Ecological Effects Test Guidelines (OPPTS 850.1075 Fish Acute Toxicity Test, Freshwater and Marine).
  • Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms, 5th Edition (EPA/821/R-02/012).

2.2.2         Data Analysis

 

 

All toxicity testing results will be analyzed using the software program ToxCalc (Version 5.0).   This program determines if there is a statistically significant reduction in response at the p = 0.05 level and utilizes the flowchart for statistical analysis outlined in EPA/821/R-02/013. The testing compared the sample responses with the laboratory

BES# 27649                                                                                                            Page 3 of 6

 

 

control water. The parameters of interest for the effluent tests are the Lethal (LC) and Inhibition (IC) Concentrations. The LC/IC values will show the point estimate of the toxicant concentration that causes a given percent reduction.

 

  1. 4.      TESTING PARAMETERS

 

Table 1: Summary of Testing Parameters

 

TestConditions

Organism

0. mykiss

Temperature

12 ± l °C

Light Intensity

50-100 ft-c

Photoperiod

16hr 1ight: 8hr dark

Test Chamber

6L aquarium

Test Solution Volume

SL

Organism Age

juvenile

Organism source

Trout Lodge

Organisms/chamber

10

Chamber/concentration

2

Organisms/concentration

20

Feeding Frequency

None

Cleaning

None

Aeration

None, unless DO falls below 6.0 mg/L

Reference Toxicant

SDS

Ref tox concentrations

8, 16, 24, 32 and 40 mg/L

Test Duration

96 hr

EffectsMeasured

Survival

TestAcceptability

2:: 90% survival in controls

 

 

BES# 27649                                                                                                            Page 4 of 6

 

 

 

  1. 5.     RESULTS

 

5.1    Oncorhynchus myldss END POINT VALUES -

 

Table 2: Range Finding Test Results

Concentration (mg/L)

Survival (%)

Cl

100

I

100

10

100

100

100

1,000

100

2,000

100

 

5.2                                                                                                                                       Testing Notes

 

 

The Laboratory control water passed the test acceptability criteria (2:,90% survival).

 

 

The survival LC50 value was> 2000 mg/L.

 

 

 

6 CONCLUSION

 

  • 0. mykiss survival was not adversely effected by the product at any of the test concentrations ofDurasoil™. The LC50 ofDurasoil™ for this test organism is

>2000 mg/L

 

 

BES# 27649                                                                                                            Page 5 of 6

 

BLOCK ENVIRONMENTAL SERVICES

96 Hr Acute Static Test Data Sheet

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