BES - Aquatic Toxicity Testing Results for Soilworks - One Product LC-50 Bioassay

Block Environmental Services, Inc.
2011-06-06
Soilworks, LLC

BLOCK ENVIRONMENTAL SERVICES

 

2451 Estand Way

Pleasant Hill, CA 94523-3911 (925) 682-7200 FAX 686-0399

 

 

 

Aquatic Toxicity Testing Results for Soilworks. l,l,C One Product LC-50 Bioassay

 

 

 

 

Prepared for:

Chad Falkenberg

Soilworks, LLC

2450 S. Gilbert Rd.• Ste 210

Chandler, Arizona 85286

 

 

 

BES Sample # 27649

 

 

 

 

Prepared By:

Block Environmental Services, Inc.

2451 Estand Way

Pleasant Hill, CA 94523

 

 

 

June 6. 2011

 

 

David Block, Ph.D              Laboratory Director


Nanette Bradbury. B.S.       Laboratory Manager

continue reading...

 

  1. 1.   INTRODUCTION

 

 

Soilworks of Chandler. Arizona requested Block Environmental Services (BES) to perform a 7 day chronic LC-50 bioassay on the synthetic dust control agent, Durasoil, using the mysid shrimp (Americomysis bahia).

 

BES received the product on April 13, 2011 and performed a multi-concentration toxicity test in order to determine a concentration-response curve for the mortality, LCS0 and IC25 endpoints.

 

The mull-concentration toxicity test consisted of five product concentrations (2000 mg/L, 1000 mg/L, 100 mg/L. IO mg/L. and 1 mg/L) and a laboratory control. run in eight replicates per concentration.

 

This report describes the procedures used and the results obtained for the aquatic toxicity test initiated on May 19, 2011.

 

BES is an Environmental Laboratory Accreditation Program certified laboratory (# 1812).

 

 

BES#  27649                                                                                                               Page 2 of 6

 

 

  1. 2.    MATERIALS AND METHODS

2.1    SAMPLE AND TEST PREPARATION Sample identification - Durasoil™

Chemical name: Synthetic Organic Dust Control Agent Appearance and odor: Colorless. odorless

Solubility: Insoluble in water

 

 

The sample was received on April 13. 2011 and stored at ambient room temperature for the duration of the study.

 

Samples to set up the definitive tests were weighed out using a Sartorius Balance, disposable dispen:.er pipettes and clean glassware.

 

A summary of testing parameters by organism can be found in Table 1.

 

 

2.2    TOXJCITY TEST PROCEDURES

 

 

2.2.1         Test Procedures - A detailed procedure for this test is outlined in laboratory standard operating procedures (SOPs) kept at the BES laboratory and they are based upon the following reference:

 

  • EPA l-s:cological Effects Test Guidelines (OPPTS 850.1350 Mysid Chronic Toxicity Test)
    • Shon-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms (EPA/821/R-02/014.

 

2.2.2         Data Analysis

 

 

All toxicity testing results ,vere analyzed using the software program ToxCalc (Version  5.0). The software determines if there is a statistically significant reduction in response at the p Cc'.

0.05 level and uti Iizes the flowchart for statisticaI analysis outlined in EPA/82 l /R-02/01 3.

 

BES# 27649                                                                                                             Page 3 of 6

 

 

 

 

The testing compared the sample responses with the laboratory control water. The parameters of interest for the effluent tests are the Lethal (LC) and lnhibilion (IC) Concentrations. The LC/IC values will show the point estimate of the toxicant concentration that causes a given percent reduction.

 

 

3, TESTING PARAMETERS

 

Table I: Summary of Testing Parameters

Test Conditions

Organism

A. b'1hia

Temperature

25    1 °C

Light Intensity

10-20 µE/m2/s

Photopcriod

16hr light: 8hr dark

Test Chamber

400 ml beaker

Test Solution Volume

200ml

Organism Age

7 days

Organism source

Aquatox

Organisms/chamber

5

Chamber/concentration

8

Organisms/concentration

40

Feeding Frequency

Twice/day

Cleaning

Siphon daily

Aeration

None. unless DO falls below 4.0 mg/L

Reference Toxicant

K2CrO4

Ref tox concentrations

16.9, 8.5, 4.2, 2.1, and 1.05 mg/L

Test Duration

7 days

Effects Measured

Survival and Growth

Test Acceptability

:::, 80% survival in controls; :::. 0.20 mg average weight of controls

 

 

-

 

 

 

BES# 27649                                                                                                              Page 4 of 6

 

 

  1. 4.     RESULTS

 

4.1    

Treatment

(%)

7 Day Sundval

7 Day Dry Weight/Organism

Average(%)

Standard Dev.

Average ( ml!)

Standard Dev.

Laboratory Control

93

0.52

0.31

0.05

I

95

0.46

0.27

0.04

10

88

0.74

0.28

0.05

100

88

0.74

0.24*

0.04

toOO

90

0.76

0.25

0.09

2000

78

0.64

0.20*

0.04

 

 

A. bahia END POINT VALUES- Raw Daat   S ummary

 

 

 

 

 

 

 

 

* Statistically significant reduction compared to the Laboratory control (p"' 0.05)

 

Staf1sf1caI Ana1Iy. s1s S ummary

End Point

% Durnsoil

Survival                          LC 50

>2000

NOEC

:woo

LOEC

>2000

Growth                           IC 25

1300

NOEC

10

LOEC

100

 

4.1.1                                                                                                                                                    Testing Notes

 

The Laboratory control water passed both the survival( 80%) and growth( 0.20 mg/surviving adult) test acceptability criteria.

 

The survival LC50 and growth IC25 values were hoth > 1000 mg/L.

 

 

 

 

 

BES# 27649                                                                                                              Page 5 of 6

 

  1. 5.   CONCLUSIONS

 

The objective of this test was to evaluate potential toxicological effects (if any) ofthe product, Durasoil™ on the mysid shrimp. A. bahia. The results of the toxicity testing with the product Durasoil™ indicate that:

A. lwhia  survival was not adversely effected by the product at any of the test concentrations of Durasoil™. Grov.-th was adversely affected at the 100 mg/L, and

2000 mg/L concentrations. The LCS0 and IC25 values for this test organism are > 2000 mg/Land> 1000 mg/L respectively.

 

 

 

BES# 27649                                                                                                             Page 6 of 6

 

 

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