Soilworks products are the industry’s top standard due to our insistence on creating high performance soil stabilization and dust control products that stand up to rigorous testing – both in the lab and in the field. Our commitment to quality and performance has led to our involvement and testing in hundreds of real-world situations. The following library of reports, presentations, specifications, approvals and other similar documents provide you, our customer, the transparency and dependable assurance that is expected from Soilworks.
This EPA-certified laboratory report evaluates the chronic aquatic toxicity of Durasoil, a synthetic organic fluid from Soilworks, through a 7-day LC50 bioassay using Americamysis bahia (mysid shrimp). The study found that Durasoil exhibited no significant toxicity to mysid shrimp at concentrations up to 2,000 mg/L. Survival rates remained high across all concentrations, with the LC50 for survival recorded at >2,000 mg/L, indicating an extremely low toxicological risk. While a minor reduction in growth was statistically significant at higher concentrations (100 mg/L and 2,000 mg/L), the IC25 remained above 1,000 mg/L—well within safe exposure limits.
These results validate Durasoil as a non-toxic dust suppressant suitable for use near marine and estuarine ecosystems, especially in compliance-sensitive or environmentally protected zones. Its insolubility in water, lack of odor, and non-volatile formulation make it ideal for projects requiring minimal ecological disturbance. For construction, remediation, or coastal operations seeking to meet stringent environmental criteria, Durasoil offers a scientifically verified, low-impact solution. Contact Soilworks to access certified toxicity documentation or for help in deploying Durasoil in sensitive aquatic environments.
BLOCK ENVIRONMENTAL SERVICES
2451 Estand Way
Pleasant Hill, CA 94523-3911 (925) 682-7200 FAX 686-0399
Aquatic Toxicity Testing Results for Soilworks. l,l,C One Product LC-50 Bioassay
Prepared for:
Chad Falkenberg
Soilworks, LLC
2450 S. Gilbert Rd.• Ste 210
Chandler, Arizona 85286
BES Sample # 27649
Prepared By:
Block Environmental Services, Inc.
2451 Estand Way
Pleasant Hill, CA 94523
June 6. 2011
David Block, Ph.D Laboratory Director
Nanette Bradbury. B.S. Laboratory Manager
Soilworks of Chandler. Arizona requested Block Environmental Services (BES) to perform a 7 day chronic LC-50 bioassay on the synthetic dust control agent, Durasoil, using the mysid shrimp (Americomysis bahia).
BES received the product on April 13, 2011 and performed a multi-concentration toxicity test in order to determine a concentration-response curve for the mortality, LCS0 and IC25 endpoints.
The mull-concentration toxicity test consisted of five product concentrations (2000 mg/L, 1000 mg/L, 100 mg/L. IO mg/L. and 1 mg/L) and a laboratory control. run in eight replicates per concentration.
This report describes the procedures used and the results obtained for the aquatic toxicity test initiated on May 19, 2011.
BES is an Environmental Laboratory Accreditation Program certified laboratory (# 1812).
BES# 27649 Page 2 of 6
2.1 SAMPLE AND TEST PREPARATION Sample identification – Durasoil™
Chemical name: Synthetic Organic Dust Control Agent Appearance and odor: Colorless. odorless
Solubility: Insoluble in water
The sample was received on April 13. 2011 and stored at ambient room temperature for the duration of the study.
Samples to set up the definitive tests were weighed out using a Sartorius Balance, disposable dispen:.er pipettes and clean glassware.
A summary of testing parameters by organism can be found in Table 1.
2.2 TOXJCITY TEST PROCEDURES
2.2.1 Test Procedures – A detailed procedure for this test is outlined in laboratory standard operating procedures (SOPs) kept at the BES laboratory and they are based upon the following reference:
2.2.2 Data Analysis
All toxicity testing results ,vere analyzed using the software program ToxCalc (Version 5.0). The software determines if there is a statistically significant reduction in response at the p Cc’.
0.05 level and uti Iizes the flowchart for statisticaI analysis outlined in EPA/82 l /R-02/01 3.
BES# 27649 Page 3 of 6
The testing compared the sample responses with the laboratory control water. The parameters of interest for the effluent tests are the Lethal (LC) and lnhibilion (IC) Concentrations. The LC/IC values will show the point estimate of the toxicant concentration that causes a given percent reduction.
3, TESTING PARAMETERS
Table I: Summary of Testing Parameters
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BES# 27649 Page 4 of 6
4.1
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A. bahia END POINT VALUES- Raw Daat S ummary
* Statistically significant reduction compared to the Laboratory control (p”‘ 0.05)
Staf1sf1caI Ana1Iy. s1s S ummary
|
End Point |
% Durnsoil |
|
Survival LC 50 |
>2000 |
|
NOEC |
:woo |
|
LOEC |
>2000 |
|
Growth IC 25 |
1300 |
|
NOEC |
10 |
|
LOEC |
100 |
4.1.1 Testing Notes
The Laboratory control water passed both the survival( 80%) and growth( 0.20 mg/surviving adult) test acceptability criteria.
The survival LC50 and growth IC25 values were hoth > 1000 mg/L.
BES# 27649 Page 5 of 6
The objective of this test was to evaluate potential toxicological effects (if any) ofthe product, Durasoil™ on the mysid shrimp. A. bahia. The results of the toxicity testing with the product Durasoil™ indicate that:
A. lwhia survival was not adversely effected by the product at any of the test concentrations of Durasoil™. Grov.-th was adversely affected at the 100 mg/L, and
2000 mg/L concentrations. The LCS0 and IC25 values for this test organism are > 2000 mg/Land> 1000 mg/L respectively.
BES# 27649 Page 6 of 6
