BES - Aquatic Toxicity Testing Results for Soilworks - One Product LC-50 Bioassay Using Pimephales promelas

Block Environmental Services, Inc.
2011-06-16
Soilworks, LLC

BLOCK ENVIRONMENTAL  SERVICES

 

 

2451 Estand Way

Pleasant Hill, CA 94523-3911

(925) 682-7200 FAX 686-0399

 

 

 

 

Aquatic Toxicity Testing Results for Soilworks, LLC One Product LC-50 Bioassay using Pimephales promelas

 

 

 

 

Prepared For: Chad Falkenberg Soilworks, LLC

2450 S. Gilbert Rd., Ste 210

Chandler, Arizona 85286

 

 

 

BES Sample # 27649

 

 

 

 

Prepared By:

Block Environmental Services, Inc.

2451 Estand Way Pleasant Hill, CA 94523

 

 

June 16, 2011

 

 

 

David Block, Ph.D 

Laboratory Director


Nanette Bradbury, B.S

Laboratory Manager

continue reading...

 

  1. 1.     INTRODUCTION

 

 

Soilworks of Chandler, Arizona requested Block Environmental Services (BES) to perform a 7 day chronic LC-50 bioassay on the synthetic dust control agent, Durasoil, using the fathead minnow (Pimephales promelas).

 

BES received the product on April 13, 2011 and performed a multi-concentration toxicity test in order to determine a concentration-response curve for the mortality, LC50 and IC25 endpoints.

 

The mult-concentration toxicity test consisted of five product concentrations (2000 mg/L, 1000 mg/L, 100 mg/L, 10 mg/L, and 1 mg/L) and a laboratory control, run in eight replicates per concentration.

 

This report describes the procedures used and the results obtained for the aquatic toxicity test initiated on June 1, 2011.

 

BES is an Environmental Laboratory Accreditation Program certified laboratory (#1812).

 

BES# 27649                                                                                                             Page 2 of 6

 

 

 

  1. 2.    MATERIALS AND METHODS

2.1    SAMPLE AND TEST PREPARATIONSample identification - Durasoil™

Chemical name: Synthetic Organic Dust Control Agent Appearance and odor: Colorless, odorless

Solubility: Insoluble in water

 

 

The sample was received on April 13, 2011 and stored at ambient room temperature for the duration of the study.

 

Samples to set up the definitive tests were weighed out using a Sartorius Balance, disposable dispenser pipettes and clean glassware.

 

A summary of testing parameters by organism can be found in Table I.

 

 

2.2   TOXICITY TEST PROCEDURES

 

 

2.2.1         Test Procedures -A detailed procedure for this test is outlined in laboratory standard operating procedures (SOPs) kept at the BES laboratory and they are based upon the following reference:

 

  • Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms (EPA/821/R-02/013).

 

2.2.2        Data Analysis

 

 

All toxicity testing results were analyzed using the software program ToxCalc (Version 5.0). The software determines ifthere is a statistically significant reduction in response at the p =

0.05 level and utilizes the flowchart for statistical analysis outlined in EPA/821/R-02/013. The testing compared the sample responses with the laboratory control water. The

 

BES# 27649                                                                                                            Page 3 of 6

 

 

 

parameters of interest for the effluent tests are the Lethal (LC) and Inhibition (IC) Concentrations. The LC/IC values will show the point estimate of the toxicant concentration that causes a given percent reduction.

 

 

  1. 3.                                                                                                                            TESTING PARAMETERS

 

Table 1: Summary of Testing Parameters

 

TestConditions

Organism

P.promelas

Temperature

25 ± I °C

Light Intensity

50-100 ft-c

Photoperiod

16hr light: 8hr dark

Test Chamber

600 mL beaker

Test Solution Volume

400ml

Organism Age

<24hr

Organism source

Aquatox

Organisms/chamber

10

Chamher/concentration

4

Organisms/concentration

40

FeedingFrequency

Twice/day

Cleaning

Siphon daily

Aeration

None, unless DO falls below 4.0 mg/L

Reference Toxicant

CuSO4 as Cu+i

Ref tox concentrations

100, 50, 25, 12.5 and 6.25 µg/L

Test Duration

7 days

Effects Measured

Survival and Growth

TestAcceptability

2". 80% survival, 2". 0.25 mg avg. dry weight/survivor in controls

 

BES# 27649                                                                                                             Page 4 of 6

 

 

 

  1. RESULTS

 

4.1    P. promelas END POINT VALVES -

 

Raw Daat   Summary

Treatment

(mg/L)

7 Day Survival

7 Day Dry Weight/Organism

Average(%)

Standard Dev.

Average (mg)

Standard Dev.

Laboratory Control

100

0

0.27

0.04

1

98

5.00

0.31

0.05

10

98

5.00

0.30

0.07

100

98

5.00

0.34

0.05

1000

93

9.57

0.34

0.05

2000

85

19.15

0.37

0.08

* Statistically significant reduction compared to the Laboratory control (p = 0.05)

 

Staf1fs1caI AnaIt.ys1sSummary

End Point

% Durasoil

Survival                         LC50

>2000

NOEC

2000

LOEC

>2000

Growth                          IC 25

>2000

NOEC

2000

LOEC

>2000

 

4.1.1                                                                                                                                             Testing Notes

 

The Laboratory control water passed both the survival( 80%) and growth( 0.25 mg/surviving adult) test acceptability criteria.

 

The survival LC50 and growth IC25 values were both> 2000 rng/L.

 

BES# 27649                                                                                                             Page 5 of 6

 

 

 

  1. 5.    CONCLUSIONS

 

 

The objective of this test was to evaluate potential toxicological effects (if any) of the product, Durasoil™ on the fathead minnow, P. promelas. The results of the toxicity testing with the product Durasoil™ indicate that:

 

P. promelas survival and growth were not adversely effected by the product at any of the test concentrations of Durasoil™.     The LC50 and IC25 values for this test organism are

> 2000 mg/L.

 

BES# 27649                                                                                                             Page 6 of 6

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