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BLOCK ENVIRONMENTAL SERVICES
2451 Estand Way
Pleasant Hill, CA 94523-3911
(925) 682-7200 FAX 686-0399
Aquatic Toxicity Testing Results for Soilworks, LLC One Product LC-50 Bioassay using Pimephales promelas
Prepared For: Chad Falkenberg Soilworks, LLC
2450 S. Gilbert Rd., Ste 210
Chandler, Arizona 85286
BES Sample # 27649
Prepared By:
Block Environmental Services, Inc.
2451 Estand Way Pleasant Hill, CA 94523
June 16, 2011
David Block, Ph.D
Laboratory Director
Nanette Bradbury, B.S
Laboratory Manager
Soilworks of Chandler, Arizona requested Block Environmental Services (BES) to perform a 7 day chronic LC-50 bioassay on the synthetic dust control agent, Durasoil, using the fathead minnow (Pimephales promelas).
BES received the product on April 13, 2011 and performed a multi-concentration toxicity test in order to determine a concentration-response curve for the mortality, LC50 and IC25 endpoints.
The mult-concentration toxicity test consisted of five product concentrations (2000 mg/L, 1000 mg/L, 100 mg/L, 10 mg/L, and 1 mg/L) and a laboratory control, run in eight replicates per concentration.
This report describes the procedures used and the results obtained for the aquatic toxicity test initiated on June 1, 2011.
BES is an Environmental Laboratory Accreditation Program certified laboratory (#1812).
BES# 27649 Page 2 of 6
2.1 SAMPLE AND TEST PREPARATIONSample identification – Durasoil™
Chemical name: Synthetic Organic Dust Control Agent Appearance and odor: Colorless, odorless
Solubility: Insoluble in water
The sample was received on April 13, 2011 and stored at ambient room temperature for the duration of the study.
Samples to set up the definitive tests were weighed out using a Sartorius Balance, disposable dispenser pipettes and clean glassware.
A summary of testing parameters by organism can be found in Table I.
2.2 TOXICITY TEST PROCEDURES
2.2.1 Test Procedures -A detailed procedure for this test is outlined in laboratory standard operating procedures (SOPs) kept at the BES laboratory and they are based upon the following reference:
2.2.2 Data Analysis
All toxicity testing results were analyzed using the software program ToxCalc (Version 5.0). The software determines ifthere is a statistically significant reduction in response at the p =
0.05 level and utilizes the flowchart for statistical analysis outlined in EPA/821/R-02/013. The testing compared the sample responses with the laboratory control water. The
BES# 27649 Page 3 of 6
parameters of interest for the effluent tests are the Lethal (LC) and Inhibition (IC) Concentrations. The LC/IC values will show the point estimate of the toxicant concentration that causes a given percent reduction.
Table 1: Summary of Testing Parameters
TestConditions |
Organism |
P.promelas |
|
Temperature |
25 ± I °C |
Light Intensity |
50-100 ft-c |
Photoperiod |
16hr light: 8hr dark |
Test Chamber |
600 mL beaker |
Test Solution Volume |
400ml |
Organism Age |
<24hr |
Organism source |
Aquatox |
Organisms/chamber |
10 |
Chamher/concentration |
4 |
Organisms/concentration |
40 |
FeedingFrequency |
Twice/day |
Cleaning |
Siphon daily |
Aeration |
None, unless DO falls below 4.0 mg/L |
Reference Toxicant |
CuSO4 as Cu+i |
Ref tox concentrations |
100, 50, 25, 12.5 and 6.25 µg/L |
Test Duration |
7 days |
Effects Measured |
Survival and Growth |
TestAcceptability |
2″. 80% survival, 2″. 0.25 mg avg. dry weight/survivor in controls |
BES# 27649 Page 4 of 6
4.1 P. promelas END POINT VALVES –
Raw Daat Summary
Treatment (mg/L) |
7 Day Survival |
7 Day Dry Weight/Organism |
||
Average(%) |
Standard Dev. |
Average (mg) |
Standard Dev. |
|
Laboratory Control |
100 |
0 |
0.27 |
0.04 |
1 |
98 |
5.00 |
0.31 |
0.05 |
10 |
98 |
5.00 |
0.30 |
0.07 |
100 |
98 |
5.00 |
0.34 |
0.05 |
1000 |
93 |
9.57 |
0.34 |
0.05 |
2000 |
85 |
19.15 |
0.37 |
0.08 |
* Statistically significant reduction compared to the Laboratory control (p = 0.05)
Staf1fs1caI AnaIt.ys1sSummary
End Point |
% Durasoil |
Survival LC50 |
>2000 |
NOEC |
2000 |
LOEC |
>2000 |
Growth IC 25 |
>2000 |
NOEC |
2000 |
LOEC |
>2000 |
4.1.1 Testing Notes
The Laboratory control water passed both the survival( 80%) and growth( 0.25 mg/surviving adult) test acceptability criteria.
The survival LC50 and growth IC25 values were both> 2000 rng/L.
BES# 27649 Page 5 of 6
The objective of this test was to evaluate potential toxicological effects (if any) of the product, Durasoil™ on the fathead minnow, P. promelas. The results of the toxicity testing with the product Durasoil™ indicate that:
P. promelas survival and growth were not adversely effected by the product at any of the test concentrations of Durasoil™. The LC50 and IC25 values for this test organism are
> 2000 mg/L.
BES# 27649 Page 6 of 6
Copyright Soilworks, LLC 2003-. All Rights Reserved. Soilworks®, Soiltac®,
Gorilla-Snot®, and Durasoil® are registered trademarks of Soilworks, LLC.
Copyright Soilworks, LLC 2003-. All Rights Reserved. Soilworks®, Soiltac®,
Gorilla-Snot®, and Durasoil® are registered trademarks of Soilworks, LLC.